Organization unit
Department of the Development of Diagnostic Tests for Use in Medicine, Veterinary Medicine, and Food Safety
Profile/Academic degree
Graduate biochemist, master biochemist
Scientific/Research title
Expert Associate
Education
Undergraduate studies: Faculty of Chemistry, University of Belgrade (2017-2021), majoring in biochemistry. Title of the final paper: “Expression and purification of recombinant M short protein SARS-CoV-2 from Escherichia coli“.
Master’s studies: Faculty of Chemistry, University of Belgrade, majoring in biochemistry. Master’s thesis title: “Differential laboratory diagnosis of mucopolysaccharidosis, presentation through patients with mucopolysaccharidosis IV-A”.
Doctoral studies: Faculty of Chemistry, University of Belgrade, Department of Biochemistry (2022-).
Qualifications
Production, purification and characterization of polyclonal antibodies; Production of recombinant proteins and monoclonal antibodies; Development and production of diagnostic tests; 2 years of work experience and participation in: Development of diagnostic test for the detection of specific IgY antibodies against bornavirus in birds; Development of universal rapid diagnostic test for the detection of antibodies specific for Trichinella spiralis in humans and animals.
Design and production of recombinant proteins in bacterial hosts, including: isolation of DNA and RNA, primer design, optimization and assembly of PCR reactions, restriction, ligation and plasmid production, chemical generation of competent cells, heat shock transformation into plasmid and protein production bacteria, minipreps, protein isolation based on 6xHis tag (Ni-based and Co-based IMAC), SDS-PAGE, Western blot and ELC etc.
Research field
She belongs to a team of researchers engaged in research in the field of diagnostic test development.
Professional engagement
Activities and other responsibilities (Training schools and scientific visits)
2023-Introductory Course for Standard Practice (GxP) organized by the Global Training Hub for Biomanufacturing (GTH-B); Seoul, South Korea
Membership in National and International Societies
Serbian Biochemical Society
Bibliography
Contact
sandra.stepanovic@inep.co.rs
Contact
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